Fig. 2

Immune cell profiling. a Immune cell distribution in pure DCIS and mixed invasive tumors. Immunofluorescence images of a pure DCIS (top left) and an area with DCIS from a mixed invasive tumor (top right). Shown below is an invasive area from a mixed invasive tumor and magnified images of an area from the periphery (i) and a central area (ii). PanCK (green), SMA (yellow), CD45 (magenta), Syto83 (DNA, blue). b In silico immune cell deconvolution of stromal segments. Each bar represents one stromal segment. The height of the colored bars represents the relative estimated abundance of the different immune cells. c Abundance of immune cell types. The percentage of estimated abundance of five selected immune cell types are shown on the y-axis and tissue type is shown on the x-axis. Boxplots illustrate the median (middle line) and the third and first quartiles (box); whiskers indicate 1.5 × IQR above and below the box. P values are from Kruskal Wallis tests